Proteomics of Biological Systems: Protein Phosphorylation Using Mass Spectrometry Techniques

Obtain protein phosphorylation knowledge using mass spectrometry techniques A practical introduction to mass spectrometry–based phosphoproteomic studies, this book addresses various concepts including the protocols necessary for sample preparation through mass spectral interpretation of protein phosphorylation events. It delves into the determining factors for whether or not a protein has been correctly modified by the addition of a phosphate group. Highlighting both basic and significant principles, as well as specific applications using examples, Proteomics of Biological Systems covers: Key fundamentals of mass spectrometry including mass analyzers, ionization, and nano–electrospray/nano–flow liquid chromatography Optimized methodologies for performing post translational modification (PTM) of protein studies using tried and tested sample preparation and analysis methods and approaches Specific examples of eukaryote and prokaryote normal state and perturbed state phosphoproteomic studies Use of the most recent systems biology bioinformatic Internet tools for interpreting complex data such as the Blast2GO gene ontology tool Step–by–step methodology for phosphoproteomic studies of both eukaryote and prokaryote systems This unique resource contains specific chapters devoted to normal state HeLa cell phosphoproteomes, DNA damage response state HeLa cell phosphoproteomes, Caulobacter crescentus normal state phosphoproteomes, and more. A core guide for scientists and researchers in academic laboratories and pharmaceutical, chemical, and biotech companies, Proteomics of Biological Systems is a useful text for expanding comprehension of mass spectrometry–based phosphoproteomic study.